Yes, you can track unique tracking pixels opens and tracking URL clicks.
When you create a new mail tracking in your dashboard, click on "Advanced settings" before you press the "Create" button. Here you will find the option "Track unique opens only", and you can toggle this setting to enable it.
Uniqueness of the tracked data is based on IP-addresses. That's why you can't track unique opens when you disable IP-address tracking within your privacy settings.
If IP-address tracking is disabled, each single open will be registered independently without verification of origin, inflating the total opens metric. Make sure IP collection is enabled if you require accurate unique open rates.